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. 2019 Aug 16;2(3):71. doi: 10.3390/mps2030071

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Representative scheme for conversion of stem cells into cancer stem cells. (A) Steps for plating induced pluripotent stem cells (iPSCs)/embryonic stem cells (ESCs) on mitomycin C-treated mouse embryonic fibroblast (MEF) feeder cells. (B) Steps for plating induced pluripotent stem cells (iPSCs)/embryonic stem cells (ESCs) on feederless gelatin-coated dishes. (C) Representative scheme for conversion of induced pluripotent stem cells (iPSCs)/embryonic stem cells (ESCs) into cancer stem cells (CSCs) in the presence of conditioned medium (CM) from cancer cell lines, and negative control without CM.