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Focused 3D classification of the TfR:Tf cryo-EM density. (A) The 7 3D classes, where nos. 1–5 have 2 Tfs bound to the TfR, while in classes 6 and 7, only 1 Tf is bound. The numbers of each class collected from the complete dataset are indicated. (B) Superimposition of the 5 classes (nos. 1–5) are shown. B, back-side view; F, front view; S, right-side view.
