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. 2019 Sep 16;7(3):115. doi: 10.3390/vaccines7030115

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Intracellular IFN-gamma staining. CD4+ and CD8a+ T cells from immunized C57BL/6 mice (4 weeks after the final injection of mRNA construct) were separated in vitro and intracellular IFN-gamma staining was separately performed in each cell population. (A) Based on the flow cytometry analysis, the booster dose group clearly has more potential to stimulate the production of IFN-gamma cytokine in the CD4+ T cell population. (B) The results for the CD8a+ cells are similar to those for the CD4+ cell population, indicating that the booster dose has a stronger effect than the single dose. As a control, we used naive splenocyte cells from the negative control group. The data was analyzed using the log-rank test (* P < 0.05; ** P < 0.01; and *** P < 0.001) and presented as mean ± SD. Error bars indicate standard deviation (SD).