Table 1.
Comparison of recovery ratio between developed method (HA vortex) and liquid collector.
Trial | Method | Concentration of Viruses in the Bubbled Water Sample (Copies/mL Water) | Dissipated Water Volume (mL) | Air Volume (m3) | Captured Viruses per Air (Copies/m3 Air) | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Qβ | PV | MNV | Qβ | PV | MNV | |||||||
Before | After | Before | After | Before | After | |||||||
1 | HA vortex | 2.9 × 108 | 2.7 × 108 | 2.5 × 106 | 1.6 × 106 | 7.7 × 105 | 1.5 × 106 | 2.06 | 121 | 1.3 × 102 | 2.5 × 100 | 1.9 × 103 |
Liquid Collector | 4.33 | 375 | 6.7 × 101 | 2.1 × 101 | 3.2 × 102 | |||||||
2 | HA vortex | 3.1 × 108 | 3.3 × 108 | 2.4 × 106 | 1.6 × 106 | 1.4 × 106 | 7.2 × 105 | 2.01 | 121 | 5.4 × 101 | 9.5 × 100 | 3.9 × 102 |
Liquid Collector | 3.52 | 375 | 4.9 × 101 | 2.3 × 101 | 1.6 × 102 | |||||||
3 | HA vortex | 3.3 × 108 | 2.9 × 108 | 2.4 × 106 | 2.1 × 106 | 1.4 × 106 | 7.2 × 105 | 2.02 | 121 | 3.5 × 101 | 1.2 × 101 | 3.5 × 102 |
Liquid Collector | 4.37 | 375 | 5.5 × 101 | 2.1 × 101 | 2.8 × 102 |
Note: Qβ; Bacteriophage, PV; Poliovirus, MNV; Murine norovirus, Before/After; Virus concentration of the virus-inoculated water sample before/after bubbling (virus generation) manipulation.