Table 2.
Pathology score conferred by immunization with vaccines in hamsters.
| Vaccine Formulation a | Mean Pathological Score b | |||
|---|---|---|---|---|
| Antigen | Adjuvant | Lung | Liver | Kidney |
| PBS | LMQ | 0.8 ± 1.1 | 1.0 ± 1.4 | 0.8 ± 0.5 |
| Heat-killed Leptospira | Freund’s | 0.6 ± 0.6 | 1.0 ± 0.0 | 0.0 ± 0.0 * |
| LigAc | LMQ | 0.6 ± 0.9 | 0.4 ± 0.6 | 1.2 ± 0.8 |
| LenA | LMQ | 1.4 ± 1.3 | 1.0 ± 0.7 | 0.4 ± 0.9 |
| LcpA | LMQ | 2.0 ± 1.2 | 1.2 ± 0.4 | 0.4 ± 0.6 |
| Lsa23 | LMQ | 1.2 ± 0.8 | 0.8 ± 0.5 | 1.0 ± 0.7 |
| LigAc, LenA, LcpA, Lsa23 | LMQ | 1.2 ± 0.6 | 1.2 ± 0.6 | 1.0 ± 1.0 |
a PBS: phosphate buffer saline; LMQ: a combination of neutral liposome, monophosphoryl lipid A, and Quillaja saponaria fraction 21. LigAc, LenA, LcpA, Lsa23: recombinant factor H binding protein subunit vaccines. The final volume ratio of LMQ or Freund’s adjuvants to immunogen was 6:4 or 1:1, respectively. b The histopathological scores were determined in all hamsters (n = 5/group) by a pathologist with blinding protocol. Statistical values of pathological score between adjuvant control group and other vaccination groups were analyzed by Mann–Whitney U-Test; * represents p < 0.05.