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. 2019 Jun 27;8(3):90. doi: 10.3390/pathogens8030090

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Characterization of mucosal-associated invariant T (MAIT) cells and the effect of S. aureus in the tonsils. A comparison of CD3⁺ MAIT and non-MAIT cells in blood and tonsil tissue in patients identified as culture positive (filled circle) or negative (open circle) for S. aureus. MAIT cells were identified as CD3⁺ CD161⁺⁺Vα7.2⁺ (A) and the remaining CD3⁺ population classified as non-MAIT cells. These cell populations were quantified in blood or tonsil tissue (B) and then assessed for CD4 and CD8 (C), CD69 (D), TCR Vβ2 (E), and TCR Vβ13.1 (F). Values are expressed as a percentage of CD3⁺ cells. Each point is a value from an individual patient and the horizontal line is the median. The two patient groups were compared with a two-tailed Mann–Whitney test or a Kruskal–Wallis test with Dunn’s multiple comparison test applied.