Fig. 5.

NKX2-1 represses gastrointestinal gene expression in AT1 cells. (A) RNA-seq comparison showing up-regulation of GI genes upon Nkx2-1 deletion in both P5 sorted GFP-marked AT1 cells averaged over 3 littermate lung pairs and P14 whole lungs from 1 littermate pair. (B) Confocal images of immunostained sections from littermate lungs showing ectopic GI markers (PIGR and TFF2) in the alveolar region of the Nkx2-1 mutant lung. Three pairs of littermate lungs were examined with consistent results. (C) Scanning electron microscopy (Top 2 rows) and transmission electron microscopy (Bottom row) of littermate lungs showing aberrant apical microvilli-like structures (arrowheads). (Scale bar: 10 µm in B; 1 µm in C.)