Fig. 1.
Inducible overexpression of TDP1 in bloodstream forms. (A) The cell line POT is a derivative of PL1S (36), in which a tetracycline-dependent overexpression construct for TDP1::TY1 was integrated in an rDNA spacer locus. (B) Western blotting analysis of TDP1 protein after 24 and 48 h of overexpression in 2 POT clones. Time point 0 h indicates noninduced condition and shows no TDP1::TY1 protein. Each lane corresponds to lysates from 1 × 106 cells. (C) Growth curve of POT1 before (Tet−, black curve) or after (Tet+, gray curve) induction of overexpression. Three independent experiments were analyzed and are represented as mean ± SEM.