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. Author manuscript; available in PMC: 2019 Oct 12.
Published in final edited form as: Environ Microbiol. 2018 Oct 30;20(11):3964–3979. doi: 10.1111/1462-2920.14421

Fig. 3.

Fig. 3.

MoPpe1 interacts with MoSap1. A. Yeast two hybrid assays for the interaction between MoPpe1 and MoSap1. The AD-MoPpe1 and BDMoSap1 vectors were co-transformed into yeast strain AH109, the transformants were plated with serial dilutions of yeast cells on SD-Leu-Trp for 3 days and on selective SD-Leu-Trp-His-Ade for 10 days and photographed. B. Co-IP assays. Total proteins extracted from Guy11, MoPpe1-Stag and MoSap1-GFP strains. Proteins were eluted from anti-GFP agarose and detected with the anti-Stag and anti-GFP antibodies respectively. Proteins in the supernatant and total proteins served as controls.