Fig. 4.

ΔMosap1 and ΔMoppe1 ΔMosap1 double mutants exhibit reduced pathogenicity. A. Two-week-old rice seedlings were sprayed with a conidial suspension (5 × 10⁴ conidia/ml) and examined at 7 dpi. Separated barley leaves were drop-inoculated with conidial suspensions and examined at 5 dpi. Experiments were repeated three times all showed similar results. B. Lesions were measured at 7 dpi, numbers within an area of 1.5 cm² were counted, quantification of lesion types as described before and error bars represent the standard deviations. Asterisks denote statistical significances (p < 0.01). C. Conidial suspensions of three different strains were injected in separate rice sheaths at 36 hpi. Sample size, 50 (n = 50), asterisks indicate IH extended to surrounding cells. Bar = 5 μm. D. Percentages of different types of infectious hyphae in rice cells, error bars represent the standard deviations.