Fig. 3.

IB analyses of hepatic P450s from cultured WT and gp78-KO mouse hepatocytes. (A) IB analyses of a representative mouse sample. (B) Densitometric quantification of the approx. 50- to 55-kDa P450 band from hepatocytes isolated from three individual WT and gp78-KO mice, cultured and pretreated with P450-inducers. See Materials and Methods for details. Values (mean ± S.D.) of three individual experiments, each employing two culture plates (technical replicates) of hepatocytes isolated from each of the three mice. Statistical significance determined by the Student’s t test, ^*,**,***P < 0.05, 0.01, and 0.001 vs. WT, respectively.