Fig. 4.

P450 functional screening using diagnostic fluorescent probes. Hepatocytes from three WT and gp78-KO mice were cultured separately, pretreated with P450-inducers, and then incubated with individual diagnostic functional probes at time 0 hour. See Materials and Methods for details. Values (mean ± S.D.) of three individual experiments each employing two culture plates (technical replicates) of hepatocytes isolated from each of the three mice. Statistical significance determined by the Student’s t test, *^,**^,***P < 0.05, 0.01, and 0.001 vs. WT, respectively.