Fig. 5.

Relative P450 mRNA expression in cultured WT and gp78-KO mouse hepatocytes. Total RNA was extracted on day 4 from cultured hepatocytes, untreated or pretreated with P450 inducers for 3 days. Hepatic P450 mRNA expression was determined by qRT-PCR analyses as detailed in Materials and Methods. Values (mean ± S.D.) are derived from three individual cultures pretreated with P450 inducers as detailed. Statistical significance determined by the Student’s t test, *P < 0.05 vs. WT. GAPDH mRNA Ct levels (mean ± S.D.) between P450-inducer pretreated WT and gp78-KO cultured hepatocytes were as follows: βNF: 100 ± 1.92 (WT)/101.49 ± 1.75 (gp78 KO); PB: 100 ± 1.67 (WT)/100.33 ± 1.61 (gp78 KO); INH: 100 ± 1.99 (WT)/99.72 ± 1.66 (gp78 KO); DEX: 100 ± 2.19 (WT)/102.85 ± 3.94 (gp78 KO). No statistically significant differences were noted by the Student’s t test in GAPDH Ct values between WT and gp78-KO cultured hepatocytes pretreated with each of the four P450 inducers. Ct, threshold cycle.