Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Summary of the PCR product (amplicon) size for each primer pair and the corresponding genotype represented
| Primer Pairs | Band Size (bp) | Genotype |
|---|---|---|
| 1+2 | 337 | Cgt (conditional gene-trap cassette) |
| 3+4 | 200 | Wild type (WT) |
| flpF+flpR | 435 | With flpe recombinase (flpe) |
| 5+6 | 327 | Floxed (fl) |
| creF+creR | 233 | With Cre recombinase |
cre, Cre-recombinase; F, forward; R, reverse.
