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. 2019 Aug 19;31(10):2332–2352. doi: 10.1105/tpc.18.00545

Figure 10.

Figure 10.

Analysis of Primary Root Phenotype and Related Gene APA.

(A) Phenotype of root. Each line contained two seedlings.

(B) Root length was measured by ImageJ software. Box plots showing change in primary root length.

(C) and (F) Sequencing coverage of primary root–related gene SAHH and ATHB13 was visualized by Integrative Genomics Viewer software. WT, wild type.

(D) and (G) Distal transcript and total gene expression of primary root–related gene SAHH and ATHB13 were verified by RT-qPCR. Error bars represent sd from three biological replicates. WT, wild type.

(E) and (H) RNA stability assay. RT-qPCR analysis of distal transcript and total gene expression of primary root–related gene SAHH and ATHB13 in control and after 120 min of cordycepin conditions. Error bars represent sd from three biological replicates (pooling ∼10 plants per condition), and asterisks are indicative of statistically significant differences using one-way ANOVA (*P-value < 0.05; **P-value < 0.01). C0, control conditions; C120, mRNA after 120 min of cordycepin treatment; WT, wild type.