Figure 12.

Abiotic Stress Responses of the SALK_146237 and SALK_205297 and 3′ sRACE Analysis.

(A) Photographs of seedlings grown on one-half-strength MS medium or one-half-strength MS medium containing 125 mM NaCl at day 14 after the end of stratification and grown on one-half-strength MS or containing 50 nM MV at day 12. WT, wild type.

(B) and (C) Seed germination rates of the indicated genotypes grown on one-half-strength MS medium or one-half-strength MS medium containing 125 mM NaCl were quantified every day from the 2nd day to the 14th day after sowing. Three independent experiments were conducted. Forty seeds per genotype were measured in each replicate. Values are mean ± sd of three replications. WT, wild type.

(D) Cotyledon-greening percentages of the 14th day were recorded. WT, wild type.

(E) Root length was measured by ImageJ software. Error bars represent sd from three biological replicates, and asterisks are indicative of statistically significant differences using one-way ANOVA (*P-value < 0.05; **P-value < 0.01). WT, wild type.

(F) Illustration of gene constructs and 3′ RACE experiment results of AT3G47610 and AKR2. The red and blue region represent PA1 and PA2 region, respectively.