Figure 2.

alix-1 Mutants Show Enhanced Sensitivity to ABA.

(A) Representative photographs of 12-d-old wild-type (Col-0), complemented line GFP-ALIX/alix-1 (labeled as GFP-ALIX), oeHA:PYL4 (HA-PYL4), alix-1, ABA insensitive cra1 and ABA hypersensitive hab1-1 abi1-2 mutants grown in the presence of 0.5 µM ABA.

(B) Percentage of seeds germinated (radicle emergence) in the presence of 1 µM ABA at 3 d after sowing. Genotypes analyzed were as in (A).

(C) ABA-mediated inhibition of seedling establishment (emergence of the first true leaves) of the same genotypes analyzed in (A) that were grown in medium lacking or supplemented with 0.5 µM ABA.

(D) Root length measurements of seedlings germinated in the presence or absence of 0.5 µM ABA after 12 d, or in the presence of 1 µM ABA after 15 d. Genotypes were as in (A).

(E) ABA concentration (pmol/g) in shoot and roots of 14-d-old seedlings for wild-type (Col-0), GFP-ALIX and alix-1 mutant. hab1-1 abi1-2 mutants were used as the ABA hypersensitive control.

(F) Photographs of representative plants analyzed in (D).

*p < 0.05; **p < 0.01 (Student’s t test; Supplemental Data Set 2) with respect to the wild type in the same experimental conditions. In all cases, data are means of three biological replicates (n = 20 in each replicate). Error bars represent sd. MS medium was used as a control in all assays.