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. 2019 Oct 8;14:8149–8159. doi: 10.2147/IJN.S214646

Figure 7.

Figure 7

(A) APTT, (B) PT and (C) haemolytic assay of PE, PE/WG and PE/WG/TiO2. For APTT assay sample with size of 1 cm * 1 cm was added with 50 µL of platelet-poor plasma (PPP) followed by incubating with 50 µL of reagent (rabbit brain cephaloplastin) and 50 µL CaCl2 (0.025 M) to calculate the blood clotting time. For PT assay sample with size of 1 cm * 1 cm was added with 50 µL of platelet-poor plasma (PPP) followed by incubating with 50 µL of thromboplastin (Factor III) to calculate the blood clotting time. For haemolytic assay samples with size of 1 cm * 1 cm added to the mixture of citrated blood and diluted saline (4:5 v/v%) for 1 hr at 37°C. After this, the samples were centrifuged and optical density (OD) was measured at 542 nm.