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. 2019 Oct 8;14:8179–8193. doi: 10.2147/IJN.S218456

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© 2019 Li et al.

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Production of antigen-specific Ig antibodies and the sIgA antibodies in immunized mice.

Notes: Antigen-specific Ig antibodies and the sIgA antibodies against the C. psittaci Ags were induced 14 days after the final immunization in vaccinated BALB/c mice. (A) The sera were collected from immunized mice to determine antigen-specific IgG antibody levels by ELISA at dilutions of 1:200. (B) The serum IgA was detected by ELISA at dilutions of 1:200. And the nasal washes and vaginal washes collected from immunized mice were determined of the sIgA antibodies' levels by ELISA at dilutions of 1:10. Each bar indicates the mean ± SD of triplicates from 6 mice per group. *P < 0.05; **P < 0.01.

Abbreviations: sIgA, secretory IgA; PBS, phosphate-buffered saline; Ags, antigens; ELISA, enzyme-linked immunosorbent assay; SIM, simultaneous; IM, intramuscular; IN, intranasal; SD, standard deviation.