Figure 3.

Ags-specific T-cell responses in spleen.

Notes: Splenocytes from immunized mice were seeded into 24-well plates and incubated at 37°C in 5% CO₂ for 48 hrs and assayed for Ags-specific CD4⁺ T-cell response. (A) Ags-specific proliferative responses in splenocytes were induced by different immunization routes. (B) Frequency of CD4⁺/IFN-γ cells was calculated according to IFCS. (C) Frequency of CD4⁺/IL-4 cells was calculated according to IFCS. (D) Ags-specific CD4⁺ T-cell response was determined by intracellular IL-4 and IFN-γ staining. The FACS plots are representative of the mean percentages of 6 mice in each group. Each bar indicates the mean ± SD of 6 mice per group from three independent results. *P < 0.05; **P < 0.01.

Abbreviations: IFCS, intracellular flow cytometry staining; FACS, fluorescence-activated cell sorting; CD4, cluster of differentiation 4; IFN-γ, interferon gamma; IL, interleukin; SI, stimulation index; SD, standard deviation.