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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Shock. 2020 Jan;53(1):16–23. doi: 10.1097/SHK.0000000000001348

Figure 5. Phosphorylation status of band 3 in RBCs incubated with trauma sera.

Figure 5.

Phosphorylation of band 3 in healthy RBCs (type O, Rh negative) measured by flow cytometry after incubation with sera from trauma patients or healthy donors, using eosin-5-maleimide staining. The band 3 phosphorylation in RBC from trauma patients over time compared to normal controls by flow cytometry and mean fluorescence intensity. We used samples from 97 trauma patients (n, 0 hour =97, 6 hour =18, 24 hour =31 and 72 hour =14) and 35 samples from healthy donors (HD). The mean fluorescence intensity [Mean ± SD (min - max)] of band 3 phosphorylation was [4052 ± 1380 (1806–7747)] for healthy donors, [6698 ± 2911 (2761–15314)] for 0 hour, [6522 ± 3115 (2693–15138)] for 6 hour, [6338 ± 2451 (3297–12492)] for 24 hour and [5557 ± 2517 (3217–12899)] for 72 hour patients.