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. Author manuscript; available in PMC: 2020 Oct 12.
Published before final editing as: Ann Biomed Eng. 2019 Apr 12:10.1007/s10439-019-02264-7. doi: 10.1007/s10439-019-02264-7

Figure 1: EP1−/− periosteal cells form more osteoblastic colonies.

Figure 1:

Periosteal cells were isolated from WT or EP1−/− mice and plated at clonal density. Colonies were stained with Crystal Violet for CFU-F and Alkaline Phosphatase for CFU-O (A-B). Mice were injected with BrdU 2 hours before sacrificing. BrdU positive cells were measured using flow cytometry (C).

N=6 replicates per group for each assay. Error bars represent standard error of the mean. Statistical analysis was performed using paired student t-test. (*)=p<0.05 (**)=p<0.01 vs. age-matched WT.