Skip to main content
. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: Phytomedicine. 2019 Apr 12;64:152927. doi: 10.1016/j.phymed.2019.152927

Figure 4. Effect of inclusion of a synthetic TLR4 ligand on antibody immune response qualityfor squalene and polyprenol nanoemulsions in immunized mice.

Figure 4.

(a) Geometric mean (+/− geometric s.d.) of antigen-specific total IgG endpoint titers as measured by ELISA. (b) Geometric mean (+/− geometric s.d.) of antigen-specific IgG1 endpoint titers as measured by ELISA. (c) Geometric mean (+/− geometric s.d.) of antigen-specific IgG2c endpoint titers as measured by ELISA. (d) Geometric mean (+/− geometric s.d.) of IgG2c/IgG1 ratios. (e) Arithmetic mean (+/− s.d.) of antigen-specific IgG bone marrow long-lived antibody secreting cells as measured by ELISpot assay 63 days after prime immunization. (f) Arithmetic mean (+/− s.d.) Antigen-specific IgG2c bone marrow long-lived antibody secreting cells as measured by ELISpot assay 63 days after prime immunization. (g-i) Geometric mean (+/− geometric s.d.) of virus neutralizing antibodies to homologous or heterologous strains measured 35 days after prime immunization using a retrovirus pseudotype neutralization assay. *p<0.05 vs H5N1 (color of asterisk represents group, underlined asterisk represents inclusion of GLA).