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. 2019 Oct 8;14:8161–8177. doi: 10.2147/IJN.S214224

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© 2019 Chen et al.

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Biocompatibility assessment of formations. (A, B) Evaluation of cell viability (CCK-8 assay) of A549 cell lines after 48 hrs of treatment with drugs in particle form or in solution, respectively. Values are expressed as mean ± SD (n = 6). (C) Hemolytic effect was estimated by incubating 1.5% (v/v) rabbit erythrocytes with various gradient concentrations of mPEG-PCCL-B or iRGD-PEG-PCCL-B for 4 hrs at 37°C. And, physical observation of the supernatant after mPEG-PCCL-B (D) or iRGD-PEG-PCCL-B (E) addition and centrifugation was shown. Extreme values (100% and 0) are positive control (0.2% Triton X-100, D–H and E–H) and negative control (pH 7.4 PBS, D–A and E–A). Samples B–G in (D) and (E) represented the gradient concentrations (0.16 ~ 5 mg/mL) of polymers.