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. 2019 Oct 8;14:8161–8177. doi: 10.2147/IJN.S214224

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© 2019 Chen et al.

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Visualization and graph-based quantification of intracellular DOX. (A) Confocal fluorescence images of A549 cells localization of DOX 2 hrs post-incubation at the equivalent DOX dose of 0.5 μg/mL. Blue fluorescence signifies positive staining of DAPI and DOX is shown in red. Scale bar = 60 μm. (B) Quantified intensities (average optical density) of DOX signal by ImageJ ver 1.50i. Values are expressed as mean ± SD. ^## P < 0.01 vs mPEG-PCCL-B/DOX group.