Table 1.
Metabolic and hormonal parameters and rectal body temperature of control and C21-treated 5 week old male C57BL/6 mice fed with ND or HFD.
| Parameters/Mice groups | ND | ND-C21 | HFD | HFD-C21 | p value | ||
|---|---|---|---|---|---|---|---|
| Diet | Treatment | Interaction | |||||
| Total Kcal intake (2 weeks) | 64±4 | 55±5 | 130±6* | 133±7 | 0.0001 | n.s. | n.s. |
| Body weight gain (g) (terminal-initial) | 3.43±0.95 | 3.38±0.73 | 4.13±0.95 | 3.38±0.53 | n.s. | n.s. | n.s. |
| Initial body weight (g) | 17.00±1.33 | 16.88±1.19 | 16.00±1.30 | 16.75±0.56 | n.s. | n.s. | n.s. |
| Terminal body weight (g) | 20.43±0.57 | 20.29±0.81 | 20.13±0.44 | 20.13±0.44 | n.s. | n.s. | n.s. |
| Plasma FFA (nmol/µL) | 2.30±0.20 | 2.30±0.18 | 5.24±0.36* | 3.27±0.19# | 0.0001 | 0.0002 | 0.0002 |
| Plasma TAG (nmol/µL) | 1.96±0.23 | 2.20±0.34 | 4.03±0.25* | 3.30±0.18# | 0.0001 | n.s. | 0.0408 |
| Plasma insulin (ng/mL) | 0.78±0.06 | 0.73±0.06 | 2.34±0.09* | 1.33±0.19# | 0.0001 | 0.0001 | 0.0004 |
| Plasma TNF-α (pg/mL) | 35±3 | 34±4 | 72±16* | 43±4# | 0.0147 | n.s. | n.s. |
| Plasma adiponectin (ng/mL) | 4.41±0.19 | 3.60±0.55 | 2.68±0.43* | 5.24±0.68# | n.s. | n.s. | 0.0029 |
| Rectal body temperature days 12–14 (°C) | 37.01±0.19 | 37.29±0.18 | 37.39±0.13 | 38.04±0.13# | 0.0021 | 0.0087 | n.s. |
ND - Normal Diet, ND-C21 - Normal Diet on C21, HFD - High-Fat Diet, HFD-C21 - High- Fat Diet on C21
*
-significantly different from ND
#
-significantly different from HFD; data were analyzed using two-way ANOVA with Fisher’s LSD test at p<0.05.