Figure 2.
MdD1 is a downstream factor in the MdMYC2 response to S‐RNase in apple pollen tubes. (a) MdMYC2 binding elements. Potential MdMYC2 binding motifs as determined by MEME analysis. (b) The candidate gene expression levels were investigated by qRT‐PCR. Expression of candidate genes which expressed in pollen tube under S 1 +S 2 ‐RNase and S 3 +S 9 ‐RNase‐treated pollen tubes. Screening target genes, which strongly up‐regulated expression in pollen tubes, in response to both S 1 +S 2 ‐RNase and S 3 +S 9 ‐RNase. (c) Expression of MdMYC2 target gene levels was investigated by qRT‐PCR in S 1 +S 2 ‐RNase and S 3 +S 9 ‐RNase treated with or without MdMYC2 silencing using antisense oligonucleotide MdMYC2 (as‐MdMYC2). (d) Structure analysis of MdD1.