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. 2019 May 17;17(11):2184–2198. doi: 10.1111/pbi.13131

Figure 4.

Figure 4

MdD1 inhibits S‐RNase activity. (a) MdD1 expression level was investigated by qRT‐PCR. The pollen tubes were treated with as‐MdD1, s‐MdD1 and cytofection, respectively. as‐MdD1 is phosphorothioated antisense oligodeoxynucleotide of MdD1, s‐MdD1 is phosphorothioated sense oligodeoxynucleotide of MdD1, cytofection is transfection agent buffer, and control is the untreated pollen tubes. Data from three biological replicates were combined using a linear mixed‐effects model. The final data were normalized to the expression levels in untreated pollen tubes (control). (b) Pollen tube length in differently treated samples. (c) Inhibition of S‐RNase activity by various amounts of MdD1. Recombinant GST‐tagged MdD1 protein and His‐tagged S‐RNase protein were expressed in E. coli. S‐RNase (30 μg/mL) protein was incubated with different concentration MdD1 protein, respectively. S‐RNase activity was measured using torula yeast RNA as the substrate. Data are the mean ± SEM. *P < 0.05. (d) Pollen tube length in differently treated samples. Data are the mean ± SEM. At least 60 pollen tubes were measured.