Figure 4.

The TRP-dependent menthol response operates via CIV neurons. Silencing neural transmission in CIV neurons with active tetanus toxin (TNT) completely ablated the menthol response, while CIV-driven expression of inactive TNT did not significantly affect the rolling response. CIV-specific, RNAi-mediated knockdown using two independent transgenes for TrpA1 and Trpm resulted in fewer animals rolling in response to menthol, as compared to the control (TrpA1 RNAi 1, p = 0.0032; TrpA1 RNAi 2, p = 0.0018; Trpm RNAi 1, p = 0.0032; and Trpm RNAi 2, p = 0.0032). Proportions represented as % ± s.e.p. n = 30 for each condition. (Online version in colour.)