Inhibition of CD163 and CD204 expression and supernatants active TGF-β1 secretion in human macrophages by recombinant rPTX2/SAP in vitro. (A,B,C) Quantification (A) and immunohistochemical staining (B) of CD163 and CD204, and ELISA analysis of supernatants active TGF-β1 (C) in human PBMC-derived macrophages after incubation alone or with IL-6 or IL-10 in the presence or absence of rPTX2/SAP. Original magnification ×400. Scale bar, 100 µm. *, P<0.05. TGF-β1, transforming growth factor-β1; rPTX2/SAP, recombinant pentraxin 2/serum amyloid protein; PBMC, peripheral blood mononuclear cells; IL, interleukin.