Fig. 5.

MoSsb1, MoSsz1, and MoZuo1 are required for cell-wall integrity. A, Light microscopic examination of protoplast release after treatment with cell wall–degrading enzymes for 1 h at 30°C. B, Data statistics of protoplast release assay. Asterisks indicate significant differences at P < 0.01. The experiment was performed three times with similar results. C, N-acetylglucosamine (GlcNAc) determination by the fluorimetric Morgan-Elson method showed significantly decreased chitin content in the three mutants. Asterisks indicate significant differences at P < 0.01, according to Duncan’s range test.