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. 2019 Mar 12;107(8):2649–2657. doi: 10.1002/jbm.b.34354

Figure 5.

Figure 5

Detection of cartilage‐specific collage type II in constructs after 14 days of culture. Collagen type II immunostaining stain of tissue section documented the presence of proteoglycans in red. Nuclei and other ECM/bioink appear blue to purple by hematoxylin counterstain. Porcine chondrocytes were embedded in (A,B,C,G,H,I) gelatin and (D,E,F,J,K,L) hyaluronic acid bioinks with low (B,E,H,K) and high (C,F,I,L) cell density using the Cellbricks bioprinting technique. (A,D,G,J) Cell‐free constructs cultured for 14 days. (A–F) Scale bar = 500 μm; (G–L) Scale bar = 100 μm.