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. 2019 Mar 4;51(4):1420–1429. doi: 10.4143/crt.2018.638

Fig. 3.

Fig. 3.

MiR-1246 promotes epithelial-mesenchymal transition (EMT) in A549 cells. The mRNA expression of markers (E-cadherin, vimentin, and transforming growth factor β [TGF-β]) related to EMT were measured by real-time quantitative polymerase chain reaction (RT-qPCR) assay. A549 cells were transfected with miR-1246 mimics or negative control (NC), miR-1246 inhibitor, or inhibitor negative control (Inh-NC). After 48 hours, whole cell lysates were prepared and RT-qPCR assay determined the mRNA level of EMT-related markers. (A) The mRNA levels of E-cadherin, vimentin, and TGF-β in A549 cells transfected by miR-1246 mimics or NC. (B) The levels of E-cadherin, vimentin, and TGF-β mRNA in A549 cells transfected with miR-1246 inhibitor or Inh-NC. All experiments were carried out more than three times and results are expressed as mean±standard error of mean (*p < 0.05, **p < 0.01).