. 2019 Oct 3;4:43. Originally published 2019 Mar 6. [Version 2] doi: 10.12688/wellcomeopenres.15065.2

Table 2. Performance of standards in the assay.

The mean values obtained from n=7 standard curves is shown.

miRNA	r ²	Slope	Efficiency (RT&PCR)
miR-16-5p	0.9985	-4.127	75.0%
miR-155-5p *	0.9984	-4.137	75.5%
miR-155-3p *	0.9977	-4.050	77.2%

*On several occasions the lowest standard (0.0001fmol synthetic miRNA) did not give amplification above background; in such cases this point was not considered, increasing the detection limit of the assay to 0.001fmol, and the values are calculated from the remaining points.