Table 3. Copy-numbers per cell of different miRNAs obtained using the absolute quantitation approach.
Compiled data from n=4–6 human donors is shown. *For some donors, this was below the lowest detectable standard in the assay.
| miRNA | Condition | Total copies/cell | |
|---|---|---|---|
| Mean ± SEM | Range | ||
| miR-16-5p | resting | 12,496±1,984 | 5,653–17,029 |
| 2hrs LPS | 14,306±1,561 | 9,969-17,048 | |
| 24hrs LPS | 11,880±2,935 | 6,052–15,406 | |
|
hsa-miR-
155-5p |
resting | 1,315±417 | 448–2,915 |
| 2hrs LPS | 5,578±1,361 | 1,609-7,777 | |
| 24hrs LPS | 9,203±4,216 | 3,820–17,517 | |
|
hsa-miR-
155-3p |
resting | 29±11 | 1.6 *–67 |
| 2hrs LPS | 767±137 | 446–1,134 | |
| 24hrs LPS | 35±29 | 3 *–94 | |
*For some donors, this was below the lowest detectable standard in the assay.