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. 2019 Sep 5;71(10):1756–1765. doi: 10.1002/art.40939

Figure 2.

Figure 2

Survival of synovial CD4+CD45RO+PD‐1+ T cells in patients with JIA is dependent on fatty acid oxidation. A, Representative gating strategy for sorting PD‐1+ and PD‐1− CD4+CD45RO+ T cells from synovial fluid and blood. B, Frequency of viable cells relative to the number of initially seeded cells determined on day 2 and day 4 after stimulation with anti‐CD3 and anti‐CD28 antibodies in the presence or absence (control) of 200 μM etomoxir. Bars show the mean ± SEM. Data were pooled from 2 experiments (n = 3 blood and synovial fluid samples per experiment). *** = P < 0.0002 by unpaired 2‐tailed t‐test. NS = not significant. C, TWIST1 mRNA expression relative to HPRT in PD‐1+ and PD‐1− CD4+CD45RO+ T cells directly isolated from synovial fluid from patients with JIA (n = 6). * = P = 0.016 by Wilcoxon's 1‐tailed paired rank test. See Figure 1 for other definitions. Color figure can be viewed in the online issue, which is available at http://onlinelibrary.wiley.com/doi/10.1002/art.40939/abstract.