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. 2019 Oct 14;19:266. doi: 10.1186/s12935-019-0981-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Effects of compound 3, cDDP, or a combination of the two drugs on the induction of NSCLC cell apoptosis and the expression of relevant proteins in NCI-H441 cells. a Flow cytometry was used to detect cell apoptosis in NCI-H441 cells treated with various concentrations of compound 3 and cDDP in combination or individually. b, c The ratio of apoptotic cells in each group are expressed as percentages. d The expression levels of Bax and Bcl-2 were determined by western blotting after 12 h of drug treatment, using β-tubulin as the loading control. e, f Quantification of relative Bax and Bcl-2 protein levels. Data represent the average of three independent experiments (mean ± SD). Data represent the average of three independent experiments (mean ± SD). ***p < 0.001 vs the control; ^###p < 0.001, ^##p < 0.01, cDDP + compound 3 vs compound 3