. 2019 Oct 14;10:77. doi: 10.1186/s40104-019-0388-8

Table 1.

Sperm quality parameters from samples exposed to extender alone (control), or in the presence of 1,3-propanediol (PDO) at three different concentrations (0.1 mmol/L, 1 mmol/L and 10 mmol/L). The evaluated parameters were: percentage of progressively motile spermatozoa (PMOT), percentage of spermatozoa with low membrane lipid disorder (%M540⁻/YO-PRO-1⁻ sperm cells) and percentage of spermatozoa with high mitochondrial membrane potential (MMP; %JC1_agg spermatozoa). Determinations were performed in fresh (extended) and frozen-thawed (FT) spermatozoa after 30 and 240 min of thawing. Data reported as mean ± SEM

Variable	PDO concentration	Time point
Variable	PDO concentration	Extended	FT-30 min	FT-240 min
%PMOT	CNT	69.87 ± 3.20	23.38 ± 2.74^a	10.96 ± 1.12^a,b
	0.1 mmol/L		26.62 ± 2.10^a	7.60 ± 0.42^b
	1 mmol/L		22.80 ± 2.12^a	11.71 ± 1.07^a
	10 mmol/L		24.94 ± 2.20^a	8.48 ± 0.45^a,b
%M540⁻/ YO-PRO-1⁻	CNT	80.83 ± 2.79	39.03 ± 1.49^a	31.25 ± 1.06^a
	0.1 mmol/L		48.12 ± 1.75^b	37.56 ± 1.50^b,c
	1 mmol/L		53.28 ± 2.10^b	42.93 ± 1.38^c
	10 mmol/L		56.02 ± 2.32^b	36.50 ± 1.23^a,b
%JC1_agg	CNT	82.79 ± 2.41	31.95 ± 1.89^a	26.01 ± 1.44^a
	0.1 mmol/L		48.76 ± 1.85^b	38.67 ± 1.58^b
	1 mmol/L		40.95 ± 2.07^c	34.39 ± 1.54^{b, c}
	10 mmol/L		35.89 ± 2.06^{a, c}	30.56 ± 1.29^{a, c}

Different letters (^a–c) indicate significant differences (P < 0.05) between treatments within a given time point for each parameter