. 2019 Oct 14;10:77. doi: 10.1186/s40104-019-0388-8

Table 2.

Sperm quality parameters from samples exposed to extender alone (control), or in the presence of acetazolamide (AC) at three different concentrations (250 μmol/L, 500 μmol/L and 1000 μmol/L). The evaluated parameters were: percentage of progressively motile spermatozoa (PMOT), percentage of spermatozoa with low membrane lipid disorder (%M540⁻/YO-PRO-1⁻ sperm cells) and percentage of spermatozoa with high mitochondrial membrane potential (MMP; %JC1_agg spermatozoa). Determinations were performed in fresh (extended) and frozen-thawed (FT) spermatozoa after 30 and 240 min of thawing. Data reported as mean ± SEM

Variable	AC concentration	Time point
Variable	AC concentration	Extended	FT-30 min	FT-240 min
%PMOT	CNT	69.87 ± 3.20	23.38 ± 2.74^a	10.96 ± 1.12^{a, b}
	250 μmol/L		25.34 ± 2.83^a	13.47 ± 2.19^a
	500 μmol/L		22.99 ± 2.57^a	12.94 ± 1.98^{a, b}
	1000 μmol/L		20.77 ± 2.29^a	8.93 ± 1.30^b
%M540⁻/ YO-PRO-1⁻	CNT	80.83 ± 2.79	39.03 ± 1.49^{a, b}	31.25 ± 1.06^{a, b}
	250 μmol/L		40.26 ± 1.73^{a, b}	27.01 ± 0.98^a
	500 μmol/L		46.39 ± 1.30^a	35.28 ± 1.71^b
	1000 μmol/L		36.03 ± 1.58^b	30.52 ± 1.18^{a, b}
%JC1_agg	CNT	82.79 ± 2.41	31.95 ± 1.89^a	26.01 ± 1.44^{a, b}
	250 μmol/L		29.30 ± 1.63^a	23.70 ± 1.24^{a, b}
	500 μmol/L		40.84 ± 2.11^b	31.98 ± 1.89^a
	1000 μmol/L		30.20 ± 1.72^a	21.92 ± 1.35^b

Different letters (^{a, b}) indicate significant differences (P < 0.05) between treatments within a given time point for each parameter