Fig.3.

Alteration in gene expression and protein production under simulated microgravity. A. VIM as a component of the cytoskeleton showed significantly up-regulation in the both attached cells and spheroids, after five days (3.3x and 3.6x respectively, *P<0.05 each). VIM up-regulation was accompanied by increased vimentin protein production, B. RHOA also showed significant up-regulation in the both adherent cells and spheroids, under simulated microgravity (3.2x and 3.9x respectively, *P<0.05 each). In contrast, RhoA protein content was not increased under simulated microgravity, and C. BRCA1 showed significant up-regulation in the both adherent cells and spheroids (2.1x and 4.1x respectively, *P<0.05 each). BRCA1 protein content was not significantly increased, as shown by western blot.