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. 2019 Sep 8;22(1):9–16. doi: 10.22074/cellj.2020.6302

Fig.2.

Fig.2

The expression of the LC3 protein in in vitro matured MII oocytes, isolated from aged and young mice was determined by the Immunofluorescence staining. A. The micrograph represents a significant difference in intensity of the LC3 expression between the young MII oocyte, young MII oocyte+melatonin, aged MII oocyte+melatonin, and aged MII oocyte groups. The nuclei were stained by DAPI. The secondary antibody was conjugated with FITC (magnification ×400, scale bars: 20 µm) and B. Significantly higher levels of LC3 were found in the aged MII oocyte+melatonin compared with the aged MII oocyte groups (P<0.01). The expression of the LC3 was significantly higher in the young MII oocyte+melatonin than the young MII oocyte groups (P<0.01). Y+M; Young MII oocyte+melatonin and O+M; Aged MII oocyte+melatonin.