Table 2.

Effect of the Super-Agrobacterium ver.1, ver.3, and ver.4 on plant regeneration and transformation of the ‘Micro-Tom’ cotyledons.

		Numbers of						Transformation frequency
Experiment repetition	Agrobacterium strain	Segments	Calli	Shoots	Rooting	Diploid	Single copy	Inoculated Segments
								/Single copy (%)
1st	C-G	110	56	26	7	5	3	2.7
	V1-G	125	94	52	47	19	12	9.6
	V3-G	92	80	152	39	14	8	8.7
	V4-G	96	84	163	59	29	14	14.6
2nd	C-G	94	49	20	15	11	6	6.4
	V1-G	88	78	88	15	23	9	10.2
	V3-G	95	72	144	40	22	11	11.6
	V4-G	75	70	157	41	20	11	14. 7
3rd	C-G	82	42	26	11	6	3	3.7
	V1-G	85	78	57	25	16	8	9.4
	V3-G	88	65	101	31	16	8	9.1
	V4-G	91	86	95	63	28	15	16.5

Each column “Segments,” “Calli,” “Shoots,” “Rooting,” “Diploid,” and “Single copy” indicated the number of occurrences. “Segments” means the number of segments used for inoculation of A. tumefaciens. “Calli,” “Shoots,” and “Rooting” showed the number of regenerate calli, shoot, and rooting. “Diploid” was the number rooting shoots that were diploid, detected by the ploidy analyzer. “Single copy” means the number of diploid rooting shoots with single copy of T-DNA, identified by Southern blot analysis. Transformation frequency was calculated as follows: the total number of transgenic plants with diploid and single copy was divided by the number of explants inoculated and then multiplied by 100. Only one plant regenerated per cotyledon explant was considered to calculate transformation efficiency. Three replications were done for this experiment.