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. 2019 Sep 29;2019:2160642. doi: 10.1155/2019/2160642

Figure 3.

Figure 3

SOD2-siRNA reversed curcumin-induced cytoprotection and SOD2 upregulation in HT22 cells exposed to OGD/R. The cells were divided into 3 groups, including control, SOD2-siRNA, and scrambled (SC)-siRNA; after 6 h incubation, western blot and MTT assay were taken to assess SOD2 expression and cell viability, respectively. Then, the cells were divided into 5 groups, including control, OGD/R, Cur + OGD/R, SOD2-siRNA + Cur + OGD/R, and SC-siRNA + Cur + OGD/R; after 3 h OGD plus 24 h reoxygenation, SOD2 expression and activity, cell viability, and lactic dehydrogenase (LDH) were assessed. (a) SOD2-siRNA inhibited SOD2 protein expression (n = 4). (b) Either SOD2-siRNA or SC-siRNA induced no obvious cytotoxicity (n = 8). (c, d) SOD2-siRNA reversed curcumin-induced effects on SOD2 expression (n = 4) and activity (n = 8). (e) SOD2-siRNA reversed curcumin-induced cell viability restoration (n = 8). (f) SOD2-siRNA reversed curcumin-induced LDH release decrease (n = 8). Results are expressed as means ± SD. ∗P < 0.05; NS: no significance.