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ALK5 and TAK1 inhibitors suppressed TGFβ-induced phosphorylation of SMAD2 and p38. Western blotting was used to examine levels of phosphorylated SMAD2 and p38 MAPK. Synovial fibroblasts were stimulated with α-MEM (control), hrTGFβ (TGFβ), hrTGFβ + SB505124 (TGFβ + SB505124), or hrTGFβ + SB203580 (TGFβ + SB230580) for 30 min prior to protein extraction and western blotting. P indicates the phosphorylated form.
