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p38 inhibitor partially suppressed TGFβ-induced VEGF mRNA and completely suppressed VEGF protein production. VEGF mRNA by RT-PCR (a) and VEGF protein concentration by ELISA (b). Synovial fibroblasts were stimulated with α-MEM (control), human recombinant (hr) TGFβ (TGFβ), or hrTGFβ + SB203580 for 6 hours prior to RT-PCR or ELISA. Values represent mean ± SE (n = 8). ∗p < 0.05 compared to control.
