Figure 1.

ANG activates ARE-dependent promoters selectively in astrocytes. A, C, and E, graphs showing that treatment with tBHQ (40 μm) increased hPAP activity by 15-fold in astrocytes (A), 7-fold in cortical neurons (C), and 249-fold in a mixed culture (E). B, D, and F, graphs showing that treatment with WT ANG (5 μg/ml) increased hPAP activity by 4-fold in astrocytes (B) and 49-fold in a mixed culture (F) but did not change the activity significantly in cortical neurons (D). hPAP activity remained unchanged upon treatment with defective ANG variants (H114R, S28N, and C39W). Significant changes in hPAP activity compared to vehicle controls are marked by asterisks, where * refers to p ≤ 0.05.