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ANG drives the expression of ARE-dependent genes in astrocytes. A and B, graphs of quantitative RT-PCR data demonstrating that treatment with tBHQ (40 μm) increased the expression of antioxidant genes encoding NQO1, GCLM, and GSTα4 in both astrocytes and cortical neurons relative to vehicle. In contrast, WT ANG promoted the expression of these genes in astrocytes (A) but not cortical neurons (B), and higher ANG concentration induced greater gene expression.
