Figure 6.

Effects of PTN and NAZ2329 on the tyrosine phosphorylation of p190RhoGAP in BHK-21 cells expressing PTPRZ or its mutant. A and B, BHK-21 cells were transfected with WT PTPRZ-B, its CS, ΔD2, or DDKK mutant, or the mock control, together with FLAG-tagged p190RhoGAP. Transfected cells were treated with 100 nm PTN or vehicle (A), or 25 μm NAZ2329 or vehicle (B) for 1 h. The Tyr-1105 phosphorylation of FLAG-tagged p190RhoGAP immunoprecipitation was assessed using anti-Tyr(P)-1105 and anti-FLAG antibodies, respectively. The scatter plot shows Tyr-1105 phosphorylation levels relative to vehicle-treated levels (n = 5 independent cell culture per group). *, p < 0.05; significantly different from the vehicle-treated control of each group by Welch's t test. Full-length blots for those that are cropped are shown in Fig. S4.