Figure 8.

Effects of the Ptprz-CS or ΔD2 knock-in mutation on the PTN- or NAZ2329-stimulated differentiation of oligodendrocyte precursors cells into mature oligodendrocytes. A–C, glial cells were prepared from WT, KO, CS, or ΔD2 brains and cultured in differentiation medium in the presence of 100 nm PTN, 25 μm NAZ2329, or vehicle for 6 days. Fixed and permeabilized cells were stained with anti-NG2 proteoglycan (a marker of oligodendrocyte precursor cells, OPCs; red) and anti-MBP (oligodendrocytes, green) antibodies in conjunction with the DAPI labeling of nuclei (blue) (A). Scale bars, 100 μm. Scatter plots show the ratio of MBP-positive cells to NG2-positive cells, in which each circle corresponds to an independent cell culture (n = 5 each). *, p < 0.05; **, p < 0.01, significantly different from WT cells under control conditions (B), or the vehicle control of each group (C) by analysis of variance with Bonferroni's post hoc tests.