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. 2019 Aug 15;294(41):14978–14990. doi: 10.1074/jbc.RA119.010323

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© 2019 Mann et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 8. — Wzt^O9a-C and Wzt^O7-C can only bind LPS from the native serotype. Purified Wzt^O9a-C (O9a CBM; A and B) and Wzt^O7-C (O7 CBM; C and D) were incubated with the O9a and O7 LPSs. Reaction mixtures consisting of 200 μg of CBM and 200 μg of LPS were mixed with nickel magnetic beads and collected using a magnet. The samples are unbound material in the supernatant (S) and material released by sequential washes of the beads in the absence (W1–W3) or presence of imidazole (E1–E3). Aliquots of the collected samples were subjected to SDS-PAGE. LPS was detected by silver staining (top panels), and protein was detected by simply blue staining (bottom panels).